D-EA p138 is consideres as diffused (EA-D) early antigen. Regarding the EA-D, it is located in nucleus and cytoplasm and it has been described the highly presence of the diffuse early antigen of EBV in lymphomas and lymphoproliferative disorders. The D-EA p138 is an antigen codified by the gene BALF2 which is the major DNA binding protein of EBV. At the time of infection with Epstein-Barr virus, antibodies to EA are found and usually last for four to six months only. The absence of antibody to EA when other EBV antibodies are present strongly suggests that first time infection with EBV occurred in the past.