D-EA p54 (p47/54) is considered as diffused (EA-D) early antigen. Regarding the EA-D, it is located in nucleus and cytoplasm and it has been described the highly presence of the diffuse early antigen of EBV in lymphomas and lymphoproliferative disorders. The D-EA p54 is an antigen codified by the BMRF1 gene, which is a DNA polymerase processivity factor. At the time of infection with Epstein-Barr virus, antibodies to EA are found and usually last for four to six months only. The absence of antibody to EA when other EBV antibodies are present strongly suggests that first time infection with EBV occurred in the past.