Custom-made services for production of recombinant antigens

For our IVD manufacturer customers that want to develop a new assay and cannot find the right raw material, we offer a complete Pack for Antigen Expression and Purification which includes design of the recombinant antigen, codon optimisation, gene fragment clonation and expression, growing scaling-up, purification to homogenety and immunological studies in Western Blot and ELISA.

We like to work closely with our customers to understand their problems and provide them with products totally adapted to their needs.

STUDY OF THE PROJECT

Study of the gene sequence, theoretical sequence analysis, selection of a heterologous expression system, selection of the optimal expression vector, design of the specic primers, codon optimization of the gene, etc.

DNA CONSTRUCTION

Amplication by PCR, clone of the target DNA in a selected expression vector, validation of the selected clone by sequencing and restriction analysis

OPTIMIZATION OF EXPRESSION LEVELS

Screening and selection of induction conditions, possibility of dierent fusion tags, solubility and expression optimization MCB and WCB stocks production, etc.

UPSTREAM PROCEDURE

Obtention of the seed by shaker and scale-up in bio-fermenter

DOWNSTREAM PROCEDURE

Design of the complete DSP, purication of the target antigen by using dierent chromatographic procedures, ultraltration, diafiltration, optimization of the formulation buffer, etc.

QUALITY CONTROL

Purity, integrity, macroaggregation and microaggregation analysis, stability, western blot analysis, ELISA assay, etc.

PROJECT INFORMATION

Keeping you constantly informed on the project progress

ANTIGEN DELIVERY

Storage in sterile labeled plastic vials at -80^oC until release. Shipment in dry ice

Service detail:

Quantity of 3 to 5 mg recombinant antigen expressed in E. coli
Purity greater than 95%, analysed by SDS-PAGE Coomassie-stained gels
Purified recombinant antigen will be delivered on dry ice
Detailed specification sheet including QC data will be provided
Antigen always avaliable for futher bulk orders at reduced price

In order to apply for our services, you should contact us by email or telephone ((+34) 958 63 70 85 / (+34) 607 86 15 73).

We ask you to fill up a questionnaire which allows us to adjust as much as possible to your requirements. Once we analyse your request, we will provide you with a quotation adapted to your inquiry. We have a commitment for complete confidentiality over all the data provided by customers and the generated data related to your project.