Fast delivery and outstanding technical support

Custom-made services for production of antigens

We offer our custom-made service of design and production of recombinant proteins to our customers that are IVD manufacturers and want to develop a new assay but cannot find the right raw material in the market. This service includes the initial design of the antigen and its optimal production in Escherichia coli or in Pichia pastoris as heterologous expression systems.

We like to work closely with our customers to understand their problems and provide them with products totally adapted to their needs.

STUDY OF THE PROJECT

Study of the gene sequence, theoretical sequence analysis, selection of a heterologous expression system, selection of the optimal expression vector, design of the specific primers, codon optimization of the gene, etc.

DNA CONSTRUCTION

Amplification by PCR, clone of the target DNA in a selected expression vector, validation of the selected clone by sequencing and restriction analysis. Possibility of using different fusion tails and different secretion peptides

OPTIMIZATION OF EXPRESSION LEVELS

Screening and selection of induction conditions, expression and solubility, MCB and WCB stocks production, for reproducibility of future lots, etc.

UPSTREAM PROCEDURE

Obtention of the seed in batch by shaker and scale-up to bio-fermenters

DOWNSTREAM PROCEDURE

Design of the complete process of purification of the target protein by using different chromatographic procedures, ultrafiltration, diafiltration, etc.

FORMULATION

Optimization of the formulation of the protein storage buffer based on the use that will be subsequently given to the required protein

QUALITY CONTROL

Purity, integrity, stability, macroaggregation and microaggregation (SEC), western blot analysis, Maldi-TOF and inmunoassays (western blot, ELISA)

PROJECT INFORMATION

Keeping you constantly informed on the project progress

PROTEIN DELIVERY

Storage in sterile labeled plastic vials at -80oC until release. Shipment in dry ice. Possibility of offering lyophilized protein.

Service detail:
  • The price of the service guarantees 3 to 5 mg of antigen expressed in E. coli
  • Purity greater than 95%, analysed by SDS-PAGE Coomassie-stained gels
  • Purified antigen will be delivered on dry ice in Europe or in dry-powder format for the rest of the world
  • Detailed specification sheet including QC data will be provided
  • The antigen will be avaliable for futher bulk orders at reduced price by increasing the required amounts
  • NON EXCLUSIVE product. The service ensures that the customer will find this antigen in the market, but the amount of the service does not ensure the exclusive use of this antigen by the customer

In order to apply for our services, you should contact us.

We ask you to fill up a questionnaire which allows us to adjust as much as possible to your requirements. Once we analyse your request, we will provide you with a quotation adapted to your inquiry. We have a commitment for complete confidentiality over all the data provided by customers and the generated data related to your project.