Recombinant proteins made in Pichia pastoris

Pichia pastoris is a methylotrophic yeast widely used in the production of recombinant proteins. This eukaryotic expression system has a series of characteristics that make it suitable for this purpose, such as: having a high growth rate, even on simple and economical substrates; it can be grown in both a flask and a fermenter, which makes it useful for both small and large scales; has two genes that encode alcohol oxidases, AOX1 and AOX2, enzymes that have a strongly inducible promoter, with AOX1 being one of the strongest and most regulated promoters available; or the stable integration of the expression plasmids into its genome.

Compared to Escherichia coli, the most used expression system for protein production, the growth process of P. pastoris is slower. However, P. pastoris is capable of generating disulfide bridges, which promotes protein folding. Its glycosylation is quite different from that which takes place in higher eukaryotic systems, so blockers should be used in the detection of IgG and IgM. It is a very suitable expression system for the production of recombinant allergens, where the detection of IgE is not altered by the glycosylation of the system. However, for other proteins of human origin or certain viruses, more sophisticated expression systems will be required.

At Rekom Biotech, we design and manufacture recombinant proteins expressed in Pichia pastoris for diagnosis of humans and animals infectious diseases and allergies.

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DISEASE/ALLERGY NAME CAT NUMBER DESCRIPTION DETAILS

Alternaria alternata (Plant rot fungus)

Alt a 1
RAL0025
Unknown

Arachis hypogaea (Peanut)

Ara h 2
RAL0040 New
Conglutin (2S albumin)
Ara h 9
RAL0049 New
Nonspecific lipid-transfer protein type 1

Artemisia vulgaris (Mugwort pollen)

Art v 1
RAL0005 Top product (Satisfaction guarantee)
Defensin-like protein
Art v 3
RAL0048
Non-specific lipid transfer protein type 1 (LTP)

Babesiosis (or piroplasmosis)

BcMSA1
RAG0020 (B. canis)
RAG0020BIOT (B. canis, biotinylated)
Merozoite Surface Antigen

Bos domesticus (Cow milk)

β-lactoglobulin
RAL0032
Beta-lactoglobulin

Canis familiaris (Dog epithelium)

Can f 1
RAL0026
Lipocalin

Canis familiaris (Dog urine)

Can f 5
RAL0014 Top product (Satisfaction guarantee)
Arginine esterase, prostatic kallikrein

Coronavirus (COVID-19) / SARS-CoV-2

S1 (RBD) COVID-19
RAG0074
S1 Receptor Binding Domain (RBD)

Dengue / Flavivirus

EDENV4
RAG0070
Envelope protein

Dermatophagoides farinae (American house dust mite)

Der f 2
RAL0013
NPC2 family

Equus caballus (Horse epithelium)

Equ c 1
RAL0022
Lipocalin

Felis domesticus (Cat epithelium)

Fel d 1
RAL0023 Top product (Satisfaction guarantee)
Uteroglobin (chain 1)

Gallus domesticus (Chicken egg)

Gal d 1
RAL0033
Ovomucoid

Lepidoglyphus destructor (Storage mite)

Lep d 2
RAL0008
NPC2 family

Leptospirosis / Leptospira interrogans

LipL21
RAG0100
The second most abundant protein
LipL32
RAG0063
Major outer membrane antigen, lipoprotein

Olea europaea (Olive tree pollen)

Ole e 1
RAL0012
Proteins similar to Ole e 1

Oral herpes / Herpes simplex virus type 1 (HSV-1)

gG1
RAG0105 New
Mature glycoprotein G (gG-1)

Parietaria judaica (Pellitory of the wall pollen)

Par j 2
RAL0020
Phospholipid transfer protein (LTP)

Platanus acerifolia (London plane tree pollen)

Pla a 1
RAL0019
Invertase inhibitor

Toxoplasmosis / Toxoplasma gondii

ChimToxo1
RAG0058 (chimera)
Multi-epitope recombinant chimeric antigen for Toxoplasmosis (SAG1 and GRA8)
p30 (SAG1)
RAG0030
Major surface antigen

West Nile virus infection (WNV)

E
RAG0065
Envelope glycoprotein