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Custom-made recombinant
proteins

We carry out the initial design of the protein and its production in Escherichia coli or Pichia pastoris

If you are a manufacturer of in vitro diagnostic tests, and you want to develop a new assay, but you cannot find the appropriate protein on the market, we offer our design and production service of custom-made recombinant proteins. This service includes the initial design of the protein and its optimal production in Escherichia coli or in Pichia pastoris as heterologous expression systems.

We like to work closely with our customers to understand their problems and provide them with products totally adapted to their needs. Do not hesitate to contact us! We will develop a custom plan to help you develop the test you are looking for.

Service detail

  • The price of the service guarantees 3 to 5 mg of protein.
  • Purity greater than 95%, analysed by SDS-PAGE Coomassie-stained gels.
  • The purified protein can be sent with dry ice (liquid form) or at room temperature (lyophilised form), depending on the destination country.
  • A detailed data-sheet including the characteristics of the recombinant protein and QC performed will be provided.
  • The protein will be avaliable for futher bulk orders at reduced price by increasing the required amounts.
  • The custom-made service project will be divided into various work milestones. Each milestone will consist of a series of phases whose development will be explained in the quotation.

Boost your laboratory with customized reagents

  1. We ensure a commitment to absolute confidentiality regarding all information received and generated related to your project.
  2. We will analyze your request to prepare a quote tailored to your needs.
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Or if you prefer...

As manufacturers, we can tailor our products to your needs.

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As manufacturers, we can adapt our products to your needs.
Contact us!

Design and production process
of tailor-made recombinant proteins

STUDY OF THE PROJECT

Study of the gene sequence, theoretical sequence analysis, selection of a heterologous expression system, selection of the optimal expression vector, design of the specific primers, codon optimization of the gene, etc.

DNA CONSTRUCTION

Amplification by PCR, clone of the target DNA in a selected expression vector, validation of the selected clone by sequencing and restriction analysis. Possibility of using different fusion tails and different secretion peptides

OPTIMIZATION OF EXPRESSION LEVELS

Screening and selection of induction conditions, expression and solubility, MCB and WCB stocks production, for reproducibility of future lots, etc.

UPSTREAM PROCEDURE

Obtention of the seed in batch by shaker and scale-up to bio-fermenters

DOWNSTREAM PROCEDURE

Design of the complete process of purification of the target protein by using different chromatographic procedures, ultrafiltration, diafiltration, etc.

FORMULATION

Optimization of the formulation of the protein storage buffer based on the use that will be subsequently given to the required protein

QUALITY CONTROL

Purity, integrity, stability, macroaggregation and microaggregation (SEC), western blot analysis, Maldi-TOF and inmunoassays (western blot, ELISA)

PROJECT INFORMATION

Keeping you constantly informed on the project progress

PROTEIN DELIVERY

Storage in sterile labeled plastic vials at -80oC until release. Shipped with dry ice as a refrigerant. Possibility of offering lyophilized protein

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