Recombinant proteins made in Escherichia coli

Escherichia coli is a bacteria widely used in the laboratory due to its growth speed and few nutritional requirements. It is frequently used in genetic and molecular biology experiments, since the structure of its genome is quite flexible, allowing the mobility of genetic material through transposons, insertion sequences, bacteriophages and plasmids.

It is the most used expression system for the production of recombinant proteins for several reasons: its genome has been known for years, which facilitates its genetic manipulation; its physiology and metabolism are perfectly known; there are several well-known vectors for protein production; it can grow quickly in very simple means, giving very high levels of production at very low cost. However, it is limited for the production of proteins that require essential post-translational modifications for their folding, immunogenicity and antigenicity.

At Rekom Biotech, we design and manufacture recombinant proteins expressed in Escherichia coli for diagnosis of humans and animals infectious diseases and allergies.

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DISEASE/ALLERGY NAME CAT NUMBER DESCRIPTION DETAILS

Acquired immunodeficiency syndrome (AIDS) / Human immunodeficiency virus (HIV)

p24
RAG0057 Top product (Satisfaction guarantee)
RAG0057BIOT (biotinylated)
Viral capsid antigen

African swine fever (ASF)

ChimASFV
RAG0048 (chimera) New
Multi-epitope recombinant chimeric antigen for African Swine Fever (ASF)

Anaplasmosis / Anaplasma phagocytophilum

p44
RAG0026
Outer membrane antigen

Arachis hypogaea (Peanut)

Ara h 9 Nonspecific lipid-transfer protein type 1

Artemisia vulgaris (Mugwort pollen)

Art v 3
RAL0006
Non-specific lipid transfer protein type 1 (LTP)

Babesiosis (or piroplasmosis)

Bc28.1
RAG0029 (B. canis)
The major member of the Bc28 multigenic family
BcSA1
RAG0012 (B. canis)
Surface antigen
ChimBc
RAG0040 (B. canis, chimera) Top product (Satisfaction guarantee)
Multi-epitope recombinant chimeric antigen for Babesia canis
ChimBg
RAG0045 (B. gibsoni, chimera) New
Multi-epitope recombinant chimeric antigen for Babesia gibsoni

Betula verrucosa (White birch pollen)

Bet v 1
RAL0011 Top product (Satisfaction guarantee)
Pathogenesis-related protein (PR-10)
Bet v 4
RAL0009
Polcalcin

Bos domesticus (Cow milk)

α-lactalbumin
RAL0031
Alpha-lactalbumin
αS1-casein
RAL0027
Casein
αS2-casein
RAL0028 New
Casein
β-casein
RAL0029
Casein
κ-casein
RAL0030 New
Casein

Candidiasis / Candida albicans

Enolase
RAG0044
Antigen corresponding to the glycolytic enzyme 2-phosphoD-glycerate hyidrolyase

Canis familiaris (Dog epithelium)

Can f 1
RAL0016 Top product (Satisfaction guarantee)
Lipocalin

Chagas / Trypanosoma cruzi

1F8
RAG0003
Calcium-binding flagellar antigen
B13
RAG0103
CA-2 surface antigen. Also known as Ag2, PEP2, TcR34
ChimChagas1
RAG0093 (chimera) Top product (Satisfaction guarantee)
Multi-epitope recombinant chimeric antigen for Chagas
ChimChagas2
RAG0094 (chimera) Top product (Satisfaction guarantee)
Multi-epitope recombinant chimeric antigen for Chagas
ChimChagas3
RAG0096 (chimera) Top product (Satisfaction guarantee)
RAG0096BIOT (biotinylated, chimera)
Multi-epitope recombinant chimeric antigen for Chagas
FRA
RAG0005
Cytoskeleton associated antigen. Also known as Ag1, JL7, H49

Coronavirus (COVID-19) / SARS-CoV-2

NP (CTD) COVID-19
RAG0071
Nucleoprotein C-terminal domain

Coxsackievirus B1 infection

VP1
RAG0028
Viral polyprotein

Cytomegalovirus infection (CMV)

ChimCMV1
RAG0109 (chimera) Top product (Satisfaction guarantee)
RAG0109BIOT (biotinylated, chimera)
Multi-epitope recombinant chimeric antigen for CMV
ChimCMV2
RAG0110 (chimera)
RAG0110BIOT (biotinylated, chimera)
Multi-epitope recombinant chimeric antigen for CMV
ChimCMV3
RAG0018 (chimera) New
Multi-epitope recombinant chimeric antigen for CMV
pp150
RAG0059 Top product (Satisfaction guarantee) New
RAG0091 Top product (Satisfaction guarantee)
Viral matrix phosphoprotein
pp28
RAG0004 New
Phosphoprotein
pp52
RAG0090 Top product (Satisfaction guarantee)
RAG0090BIOT (biotinylated)
DNA polymerase processivity subunit
pp65
RAG0016 Top product (Satisfaction guarantee)
Viral tegument phosphoprotein

Dermatophagoides pteronyssinus (European house dust mite)

Der p 10
RAL0015
Tropomyosin

Dirofilariasis / Dirofilaria immitis

ChimDiT33
RAG0014 (chimera) Top product (Satisfaction guarantee)
Multi-epitope recombinant chimeric antigen for Dirofilaria immitis

Ehrlichiosis / Ehrlichia canis

gp19
RAG0025 Top product (Satisfaction guarantee)
Glycoprotein

Epstein-Barr virus infection (EBV)

ChimEBV-EA
RAG0082 (chimera) New
Multi-epitope recombinant chimeric early antigen for Epstein-Barr Virus (EBV)
ChimEBV-VCA
RAG0081 (chimera) New
Multi-epitope recombinant chimeric viral capsid antigen for Epstein-Barr Virus (EBV)
EBNA1
RAG0007 Top product (Satisfaction guarantee)
RAG0047 Top product (Satisfaction guarantee)
Late nuclear antigen
p138
RAG0033
Early antigen
p18
RAG0049 Top product (Satisfaction guarantee)
RAG0049BIOT (biotinylated)
Viral capsid antigen
p23
RAG0002
Viral capsid antigen
p54
RAG0035 Top product (Satisfaction guarantee)
Early antigen
ZEBRA
RAG0023
Transcription factor, early antigen

Equus caballus (Horse epithelium)

Equ c 1
RAL0007
Lipocalin

Feline immunodeficiency syndrome (feline AIDS) / Feline immunodeficiency virus (FIV)

gp40
RAG0066 Top product (Satisfaction guarantee)
Transmembrane subunit of the 150 kDa envelope glycoprotein
p15
RAG0015 New
Matrix protein
p24 FIV
RAG0013 Top product (Satisfaction guarantee)
Core antigen

Feline leukemia virus (FeLV)

p27
RAG0078 New
Core antigen

Gadus callarias (Baltic cod)

Gad c 1
RAL0035
Beta-parvalbumin

Genital herpes / Herpes simplex virus type 2 (HSV-2)

gG2
RAG0087
Glycoprotein G (gG-2)

Hepatitis B

HBcAg
RAG0056
Core antigen assembled as capsid-like particles
HBeAg
RAG0062
e antigen that comprises the 10 aa pre-core sequence plus the 149-residue assembly core domain

Leishmaniasis / Leishmania infantum

K39
RAG0061 Top product (Satisfaction guarantee)
RAG0061BIOT (biotinylated)
Parasite kinesin-related antigen
KMP11
RAG0038
Kinetoplastid membrane antigen of 11 kDa

Leptospirosis / Leptospira interrogans

ChimLip1
RAG0019 (chimera) New
Multi-epitope recombinant chimeric antigen for Leptospira interrogans
ChimLip2
RAG0031 (chimera) New
Multi-epitope recombinant chimeric antigen for Leptospira interrogans
ChimLip3
RAG0076 (chimera) New
Multi-epitope recombinant chimeric antigen for Leptospira interrogans
LipL32
RAG0077
Major outer membrane antigen, lipoprotein

Lyme borreliosis (or Lyme disease)

flagellin B
RAG0054 (B. afzelii)
RAG0055 (B. burgdorferi)
RAG0072 (B. garinii)
Internal central portion of 41 kDa flagelline B protein (p41)
ospC
RAG0034 (B. garinii)
RAG0042 (B. afzelii)
RAG0043 (B. burgdorferi)
Outer membrane antigen
VlsE
RAG0022 (B. garinii, chimera)
RAG0027 (B. burgdorferi, chimera) Top product (Satisfaction guarantee)
RAG0102 (B. afzelii)
Major variable Surface antigen

Malus domestica (Apple)

Mal d 3
RAL0039
Non-specific lipid transfer protein type 1 (nsLTP1)

Mycoplasma pneumoniae infection

P1
RAG0053
Adhesin protein
P30
RAG0041 New
Adhesin protein

Neosporosis / Neospora caninum

NcGRA7
RAG0024
RAG0024BIOT (biotinylated)
Dense granule antigen GRA7

Olea europaea (Olive tree pollen)

Ole e 2
RAL0010
Profilin
Ole e 5
RAL0047
Superoxide dismutase [Cu-Zn]

Oral herpes / Herpes simplex virus type 1 (HSV-1)

gG1
RAG0017
RAG0017BIOT (biotinylated) New
Mature glycoprotein G (gG-1)

Phleum pratense (Timothy grass pollen)

Phl p 1
RAL0001
Beta-expansin
Phl p 12
RAL0004
Profilin
Phl p 5a
RAL0003
RAL0003BIOT (biotinylated)
Unknown
Phl p 5b
RAL0017
Unknown
Phl p 7
RAL0002
Polcalcin

Platanus acerifolia (London plane tree pollen)

Pla a 3
RAL0021
Non-specific lipid transfer protein type 1 (LTP)

Salsola kali (Russian thistle pollen)

Sal k 1
RAL0018
Pectin methylesterase

Severe acute respiratory syndrome (SARS-2003) / SARS-CoV

NP (CTD)
RAG0080
Nucleoprotein C-terminal domain

Syphilis / Treponema pallidum

ChimSyphilis1
RAG0046 (chimera)
RAG0046BIOT (biotinylated, chimera)
Multi-epitope recombinant chimeric antigen for Treponema pallidum
ChimSyphilis2
RAG0064 (chimera)
RAG0064BIOT (biotinylated, chimera)
Multi-epitope recombinant chimeric antigen for Treponema pallidum
TmpA
RAG0073
Membrane lipoprotein
Tpp15
RAG0009
RAG0009BIOT (biotinylated)
Membrane lipoprotein
Tpp17
RAG0008
RAG0008BIOT (biotinylated)
Membrane lipoprotein
Tpp47
RAG0010
RAG0010BIOT (biotinylated)
Membrane lipoprotein

Toxoplasmosis / Toxoplasma gondii

p29 (GRA7)
RAG0083
Dense granule antigen
p30 (SAG1)
RAG0011 Top product (Satisfaction guarantee)
Major surface antigen
p35 (GRA8)
RAG0084
Dense granule antigen

Triticum aestivum (Wheat)

Tri a 19
RAL0053
RAL0053BIOT (biotinylated)
Omega-5 gliadin, seed storage protein

Tuberculosis (TB) / Mycobacterium tuberculosis (Koch's bacillus)

CFP10
RAG0050
Culture filtrate antigen of 10 kDa
CFP10:ESAT6
RAG0060 (chimera)
Multi-epitope recombinant chimeric antigen for Tuberculosis (CFP10 and ESAT6)

Typhoid Fever / Salmonella typhi

flagellin
RAG0032
Flagella antigen
OMP
RAG0021
Outer membrane protein

West Nile virus infection (WNV)

E
RAG0001
Envelope glycoprotein